Coding

Part:BBa_K4404003:Design

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-08)


acsD, acsC and acsE from A. woodii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2405
    Illegal PstI site found at 142
    Illegal PstI site found at 1577
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2405
    Illegal PstI site found at 142
    Illegal PstI site found at 1577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2405
    Illegal XhoI site found at 2320
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2405
    Illegal PstI site found at 142
    Illegal PstI site found at 1577
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2405
    Illegal PstI site found at 142
    Illegal PstI site found at 1577
    Illegal NgoMIV site found at 2017
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2291


Design Notes

It is important to produce overhangs which flank the region for the digest with specific restriction enzymes in order to secure a robust implementation into a vector system.


Source

Ordered by IDT with the genomic background of Acetobacerium woodii.

References